What biodiversity is hiding in Northern California’s un-sampled forests?

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About This Project

Large regions of Northern California are as listed as "Un-sampled" on the CA Biodiversity Database. Lets fix that!!

Let's perform the first multi-taxa collections in un-sampled areas of Northern California including Shasta, Siskiyou, and Trinity forests.

We hypothesize that multi-taxa sampling in previously un-sampled regions of Northern California will reveal undocumented biodiversity, including potentially rare or regionally distinct fungi, soil organisms, insects, lichens, and bryophytes.

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What is the context of this research?

We know that climate change is occurring, and we estimate we are losing species that we don't even know exist yet. This sample collection effort will focus on collecting from under-sampled areas of California to document diversity that may be disappearing. The goal is long-term storage of these specimens so that future analysis will be possible, before the these areas are completely transformed by climate change.

Biodiversity Loss - Climate change is increasingly becoming the dominant factor, often acting in tandem with land-use changes. The most significant impact is observed in localized extinctions, where species are unable to shift their range to suitable climates fast enough. [1, 2, 3, 4, 5]

What is the significance of this project?

Just in the past few years it has become possible to efficiently analyze DNA from ecological samples to determine what actually exists.

DNA Extraction Methodology

Many new species are discovered this way, and its becoming more apparent that localized variants of many organisms exist that were previously assumed to be identical to neighboring organisms. These localized endemic species may disappear soon due to changes in habitats.

Samples and analysis are the first step to understanding if localized species are moving to new areas. With more data and collections, we may find means to improve forest health, mitigate crop pathogens, explain insect population changes, control invasive species, and possible breakthroughs in medical science and human and animal health.

This multi-taxa project will highlight the connections between organisms. Rather than analyzing individual collections of one type of organism, we will perform a multi-taxa biome DNA survey.

What are the goals of the project?

The goal is simple: to efficiently collect samples from under-studied taxa in under-sampled locations.

These collections will be prepped, DNA analyzed, documented, and put into long-term storage in the ATBI Warehouse. We expect to perform future additional analysis as technology improves, but we must collect from these areas soon before climate change causes habitats to be transformed.

Budget

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The goal is to efficiently sample multiple taxa from understudied areas, and provide specimens and samples to the ATBI warehouse. Its very efficient to collect many taxa at the same time (fungi, soil, plants, insects, lichens, bryophytes, etc.), so resulting output will be very high compared to traditional projects focused on single taxa.

Target funding allows for collection from 5 locations, with 20 multi-taxa sample sites per location. For each $1000 of additional funding, we will add 5 more locations, each with 20 more multi-taxa samples per location.

We will utilize freeze-drying as a primary storage technique, which we feel is the best method to preserve specimens from all taxa for long-term storage at low cost. Lyophilization better retains the original characteristics of the specimens, compared to typical heat dehydration storage. DNA samples will be taken from fresh specimens during collection.

Project Timeline

The project is planned for a 6-month period in the Summer and Fall of 2026, to collect, analyze, document, and deliver all samples to the ATBI Warehouse. Should there be additional funding, it could be extended to 2027 or later years.

Jul 31, 2026

Collection and lyophilization of samples

Sep 30, 2026

DNA analysis and documentation of samples

Nov 30, 2026

Deliver all samples to ATBI Warehouse

Meet the Team

Ken Buegeleisen
Ken Buegeleisen
President of MycoSurvey

Affiliates

Massachusetts Institute of Technology (MIT), North American Mycological Association (NAMA), Sonoma County Mycological Association (SOMA), MycoSurvey, FUNDIS
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Ken Buegeleisen

I have been the chairman of the North American Mycological Association's Foray committee for three years now, and I'm the President of my local mushroom club - the Sonoma County Mycological Association. I've lead hundreds of groups documenting and collecting fungi, wild plants, and exploring California's wild biodiversity.

The mushrooms don't exist without plants, trees, and often insects, moss, specific soil structures, geologic attributes, all have a role. The mushrooms really highlight how interconnected the web of life is. Did you know that mushrooms were considered "non-flowering plants" until the 1970's? Only 50 years ago, DNA analysis revealed that fungi are a completely different kingdom of life. This essentially makes mycology one of the last frontiers to be explored. And the best part is that ANYONE can make a contribution to the science.

I taught myself computer programming when I was 12. I currently work for MIT as software engineer. My wife and I own and operate a horse boarding ranch, and I contribute to regenerative agriculture by making compost for a local CSA. I also keep my 65 year-old hay-making equipment running to cut neighboring grasslands for fire control, and feed our horses and sheep.

I have a Bachelors in Business from Saint Mary's College, and Lifetime Teaching Credential from UC Berkeley. I spent a few years teaching computer science at Santa Rosa Junior College, then decided I'd rather do, than teach!

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