Discussion
Join the conversation!Sign In
Vickie BorowiczBackerCongratulations on reaching your goal! I look forward to following your work.Nov 18, 20160
Nathaniel BaileyBackerDo you have any idea the ploidy of wild Pilocarpus, or if the library contains regions of high repeat common to many plants? I know that recently efforts assembling the genome of wheat was hampered by these two factors. How do you plan on addressing this in your whole genome assembly?Nov 15, 20160
Daniella AllevatoResearcherHi Nathaniel, the specific species that I am working with Pilocarpus pennatifolius is not a polyploid, though there are some other species in the genus that are polyploids. I also hope to use the sequenced genome of Citrus to aid my own assembly of the Pilocarpus genome.Nov 17, 20161- Nathaniel BaileyBackerCool!! Congrats on your success and thanks for your reply!Nov 18, 20160
Thomas BrunnerBackerGood luck with your research and academic studies.Nov 03, 20160- Thomas BrunnerBackerLooks like interesting research by a competent and committed scientist. Also like the creative fundraising.Nov 03, 20160
- Daniella AllevatoResearcherThank you so much for your support! I hope my research will help contribute to many studies on Pilocarpus.Nov 03, 20160
Sumner MagruderDo you need RNA-seq analysis? Differential expression? etc?Oct 25, 20160- Daniella AllevatoResearcherHi Sumner, RNA-seq would be great for future projects, but at the moment I am hoping to do full-genome sequencing of multiple populationsOct 26, 20160
EugeneBackerHi Daniella, If this plant is so important for medicinal purposes and it is going extinct why don't we plant it here in the US?Oct 20, 20160- Daniella AllevatoResearcherHi Eugene! This plant is present in two botanical gardens in the US but it is usually housed in greenhouses. Another really interesting thing to think about is that the chemistry does vary with location, so there are many environmental factors which could be important factors in the chemistry desired.Oct 22, 20160
Christina TranBackerGood luck! What an interesting plant. I was curious about what it looked like so I looked it up -- it has beautiful flowers!Oct 19, 20160- Daniella AllevatoResearcherHi Christina! The flowers are very beautiful-- I will try and post some photos from my last time in the field. The different species of this genus have inflorescence variations in both size (10cm-1m) and color (green, yellow, red, purple).Oct 19, 20160
Dr Ashley BurrowesBackerGreat research. From anecdotal to persuasive evidence. Daniella's one big step for mankind.Oct 16, 20160- Dr Ashley BurrowesBackerThis is an example of anecdotal evidence becoming more than persuasive but compelling.Oct 16, 20160
- Dr Ashley BurrowesBackerThis is the pioneering field research that could improve the health of many fellow human beings. Thank you DaniellaOct 16, 20160
- Daniella AllevatoResearcherThank you Ashley for the support!Oct 19, 20160
Stephen BridgettBackerBest wishes for fund raising and sequencing of Pilocarpus. It is good to see medicinal plants being investigated in this way.Oct 11, 20160- Daniella AllevatoResearcherThank you for your support! It is really quite an amazing plant and used in so many ways: glaucoma, dry mouth syndrome, cystic fibrosis tests, hair loss. It will be very exciting to help learn more about this amazing plant!Oct 11, 20160
Cindy WuBackerI hadn't heard of Jaborandi until reading about your project! Very interesting. After you sequence the plant, are you planning on making the sequence available to other scientists?Sep 29, 20160- Daniella AllevatoResearcherHi Cindy! Yes, it is a very interesting plant and the main compound Pilocarpine is actually on the World Health's organization list of medicines essential for basic healthcare. I will definitly be making the raw Illumina sequence data available and it will be deposited in the National Center for Biotechnology Information Sequence Read Archive (www.ncbi.nlm.nih.gov/sra)Sep 29, 20160
