About This Project
Grass Lake Research Natural Area, home to California’s largest peat-forming fen, is a rare montane ecosystem whose fungal communities remain poorly documented. This project will assess fungal diversity using soil sampling, macrofungal collections, vouchering, and DNA barcoding to establish a public repository of fungal community data. Without this knowledge, conservation and restoration efforts may overlook key aspects of peatland biodiversity.
Ask the Scientists
Join The DiscussionWhat is the context of this research?
Peat-forming wetlands are globally important ecosystems recognized for their role in hydrological regulation, long-term carbon sequestration and as biodiversity hotspots. In California's Sierra Nevada, peatlands cover an estimated 0.2% of the total land area, yet support a disproportionate amount of biodiversity. Research on montane peatlands has primarily focused on plants, hydrology, and carbon dynamics, while fungi remain largely overlooked. Although fungi are increasingly recognized as important ecological players in boreal and alpine peatlands, minimal baseline data exist for these systems in the Sierra Nevada. Therefore, documenting fungal communities is a critical first step toward understanding the holistic picture of Sierra Nevada peatland ecology and biodiversity, as well as promoting the long-term protection of these ecologically significant habitats.
What is the significance of this project?
Grass Lake, located near Lake Tahoe in the Sierra Nevada, is Californias largest peat-forming fen. The site supports high levels of biodiversity and numerous rare or unique species of plants. Wetlands in the Sierra Nevada can serve as carbon sinks, influence hydrology, and provide refuge for specialized flora and fauna, yet their fungal communities remain insufficiently documented. Fungi may play central roles in peatland ecology, mediating litter decomposition, structuring plant communities, and influencing nutrient cycling. Data on fungal diversity in California peatlands are lacking, limiting our ability to assess how the structure and function of these communities respond to disturbance, climate change, and altered hydrological regimes. This project will generate foundational knowledge of peatland fungal diversity, create publicly available resources, and highlight fungal taxa that may serve as indicators of peatland health, supporting restoration and management efforts.
What are the goals of the project?
The goal of this project is to document the fungal diversity of Grass Lake RNA through soil sampling and systematic surveys across its diverse microhabitats. From July to October, 2026, soil samples and sporocarps will be collected and sequenced. All macrofungal collections will be accessioned in the California State University East Bay (CSUEB) HAY Fungarium, and resulting data deposited in public repositories such as iNaturalist, GenBank, and UNITE. Beyond biodiversity documentation, the project aims to integrate fungi into broader frameworks for wetland ecology by highlighting their diversity, biogeography and ecological significance. Community engagement will be prioritized with collaboration from CSUEB, the Fungal Diversity Survey and community scientists to increase awareness of montane peatland fungal diversity, build long-term monitoring capacity and strengthen all taxa-inclusive conservation efforts for these rare and important reservoirs of biodiversity.
Budget
Listed budget items will assist in the completion of this research project by providing essential support in travel, accessioning and sequencing costs.
Microfungal communities in soil samples will be sequencing using the ATBI protocol. All macrofungal specimens will be DNA barcoded using Oxford Nanopore Technologies following a streamlined and cost efficient protocol for sequencing large numbers of specimens.
All appropriate field collection gear including tackle boxes, camera gear and in situ chemical tests have already been acquired. However, additional materials such as bags and desiccant packs are needed for storing dried specimens until they are ready to be accessioned.
All specimens will be accessioned for long-term preservation and future research at the CSUEB HAY Fungarium. Funding will cover costs associated with materials and labor.
Field travel funds will be strictly allocated to gas costs for driving to and from the collection site.
Endorsed by
Project Timeline
Multi-day surveys will be conducted in the summer of 2026, from July to October. According to phenological data from iNaturalist, this is the most productive time for observable sporocarp formation at the site. Tissue sampling for sequencing and specimen accessioning will be completed by December 2026. Sequence validation, uploading to public repositories and further data analysis will be completed by March 2027. Presentations will be given throughout the duration of the project.
Oct 13, 2025
Project Launched
Jul 04, 2026
Grass Lake Soil Collection and Fungal Survey #1, soil samples sent in for sequencing and macrofungal specimens dried
Jul 18, 2026
Grass Lake Soil Collection and Fungal Survey #2, soil samples sent in for sequencing and macrofungal specimens dried
Aug 01, 2026
Grass Lake Soil Collection and Fungal Survey #3, soil samples sent in for sequencing and macrofungal specimens dried
Aug 15, 2026
Grass Lake Soil Collection and Fungal Survey #4, soil samples sent in for sequencing and macrofungal specimens dried
Meet the Team
Affiliates
Affiliates
Taylor Bright
Taylor is a mycologist and naturalist focusing on macrofungal diversity, biogeography and conservation in California. She earned her BS in Biology from the University of Central Florida where she gained research experience in restoration ecology and conservation biology. Upon moving to California, Taylor fell in love with fungi and has engaged in various mycological research projects ranging from post-fire microbial ecology in the wildland-urban interface, to metatranscriptomics of soil fungi across precipitation gradients in coastal Douglas-fir forests. She also works as a field mycologist for the California Fungal Diversity Survey, a state-funded project working to systematically document macrofungal diversity across the state of California. Taylor prioritizes community engagement in the sciences, serving on the North American Mycological Association Conservation and Stewardship Committee, and on the board of the Sonoma County Mycological Association. She educates widely about various topics in mycology and leads fungal ecology walks throughout the Pacific Northwest.
Brian Perry
Dr. Perry has been at CSU East Bay since 2013, and has been studying fungal diversity, systematics and evolution since 1995. In addition to documenting the mushrooms and other fungi of Vanuatu, Hawaii and California, Dr. Perry's lab conducts research on the assemby, dynamics and biogeography of island fungal communities, endophytic fungi of Hawaiian plants, the systematics of Mycena and allied genera and the evolution of fungal bioluminesence.
Lab Notes
Nothing posted yet.
Project Backers
- 2Backers
- 100%Funded
- $3,710Total Donations
- $1,855.00Average Donation

