Jeff Young

Jeff Young

Feb 05, 2017

Group 6 Copy 189
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Samples Prepared for Analyses

NRBS (nutrient rich buffered starter) being prepared

Hey all, we found ourselves playing the waiting game over the last few months.  Let me explain.  After this experiment went public and started getting funding on Experiment.com, we began digging deeper into our sample selection to ensure that the best possible experiment could be conducted.  After some long talks with the R&D specialist at Briess Malting Co, Bob Hansen, we came up with a sample set that, while inherently variable, we might be able to get some meaningful results.  

Stepping back a bit, when we use grain as the inoculant for sour wort, we are using a ~wild~ culture.  The variables that are in place before we ever grind the malted barley and throw it into wort are complex, numerous, and frankly, not well understood or studied.  That said, you have to start somewhere and with perhaps a thoughtful sample set, we might be able to observe something useful at the end of it all.

Long-story-short, we wanted to compare malts from different regions, from different harvest years, and from different varietals.  The "wait" we went through was due to waiting for the 2016 harvest to be malted and delivered to us so we could compare some things to the 2015 harvest.  There's actually a lot more I want to say about all of this, but I'll just post our sample preparation notes and let you see where we're at.

[Note: this document is just the lab notes from the sample preparation. These samples will now be sent out for sequencing and NMR analysis.  Those results, once returned, will be the completion of this experiment.]

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About This Project

Sour beers are likely the original beer style and have made a recent comeback in terms of popularity among craft beer enthusiasts. They are made with a bacterial and fungal mix rather than pure cultures of Saccharomyces. yeast as in typical ales and lagers. However, the suite of different microbes and their relative abundances during the course of souring and fermentation remain a mystery. We aim to map part of the sour beer microbiome and identify the organic acids these microbes produce.

Blast off!

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