Using DNA Barcoding as a Citizen Science Tool for Identification of Mushroom species of the Pacific Northwest.

Extraction protocol

1. Remove a tiny piece of gill tissue with tweezers. Use isopropyl alcohol and then lighter in between to sterilize tweezers.

2. 30 microliters 0.5 M NaOH.

3. Macerated with micropestle

4. Let stand for 10 minutes.

5. Add 150 microliters 100mM tris-HCL. Pipette mix.

6. 10 minutes at 95 celsius heat bath.

7. Centrifuge 11,000 RPM, remove supernatant.

8. Freeze if needed (-20 degrees celcius) for future use.