Methods
Summary
This project will investigate whether a bioengineered humic-binding peptide can enhance extracellular electron transfer in brackish tidal marsh sediments and reduce methane (CH₄) and hydrogen sulfide (H₂S) production. The experiment will use six replicated mesocosms (three controls and three treatments) constructed to mimic natural marsh conditions using intact sediment cores collected from Cattus Island County Park.
The project combines techniques from microbial ecology, environmental chemistry, and bioengineering, including mesocosm construction, anaerobic incubation, methane monitoring, porewater sampling, and statistical comparison of replicated treatments and controls.
Challenges
Sensor and sampling reliability: Methane measurements and porewater analyses may be influenced by sampling error or equipment limitations. Repeated measurements over time and standardized sampling procedures will be used to improve consistency.
Peptide effectiveness: The engineered peptide may not produce a measurable change in electron transfer or gas emissions. In that case, the project will still provide valuable information about the role of humic-mediated electron transfer in marsh sediments and help refine future bioengineering approaches.
Pre Analysis Plan
Mesocosms receiving the engineered humic-binding peptide will exhibit lower methane and hydrogen sulfide production and higher indicators of iron reduction than untreated control mesocosms.
Protocols
This project has not yet shared any protocols.
