Methods
Summary
Our project will investigate the impact of illegal wildlife trade on antimicrobial resistance (AMR) in endangered tortoises in Madagascar using a One Health approach. To achieve this, we will molecular biology technique.
Sample collection: Cloacal and oral swabs will be collected from trafficked tortoises rescued by conservation organizations and from wild tortoises.
Laboratory analysis: We will extract genetic materials from these samples and will detect antibiotic resistance gene by amplifying them using Polymerase Chain Reaction.
Statistical analysis: We will compare the AMR profiles between trafficked and wild tortoises to assess associations with environmental or trade-related factors.
Protocols
This project has not yet shared any protocols.
