Diagnostic procedures
Dermatophyte molecular diagnostic:
Total DNA will be extracted from all samples using the DNeasy® Blood & Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. Real time - Polymerase Chain Reaction (Real time - PCR) will be carried out as described previously (Kobylak et al., 2015) for the dermatophyte detection. One amplicon of each dermatophyte species with adequate concentrations will be sequenced with the same diagnostic primers in order to confirm the PCR results.
Mycological analysis:
In order to investigate the mycrobiota present on the skin of monkeys, the carpet technique (Mariat & Adam-Campos, 1967) will be used. Each sample will be cultivated on Agar Sabouraud Caf and Actidiona (Liofilchem, Italy), and will be incubated at 25°C for 22 days (Rodríguez, 1998; Quinn et al., 1999). The identification of the isolated fungi will be carried out based on macroscopic and microscopic morphology (De Hoog et al., 2000; Larone, 2002).
Identification of ectoparasites:
Ectoparasites will be identified by morphological characteristics through the taxonomic keys.
- Published on Jul 16, 2018
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