Methods
Summary
To establish a normal range value for the size of the heart in lions, the project will employ both thoracic radiographic measurements and a blood biomarker called NT-proBNP. We will conduct a thorough examination of the lions at three zoos in Iraq; Zaraa, Sulaimani, and Erbil, followed by anesthesia. After the lions are anesthetized, we will conduct a blood and biochemistry analysis to ensure that they are healthy. Once the criteria have been confirmed, we will conduct a thoracic radiograph during two phases—inspiration and expiration—and collect blood for a blood biomarker. This project will provide a cost-effective method for the early detection and monitoring of the heart of lions in zoos and wildlife.
Challenges
This project will encounter numerous obstacles. Beginning with anesthesia and the management of the lions, the radiograph apparatus necessitates a large cassette of film due to the large thoracic area of the lions. Additionally, the recovery process after anesthesia presents a multitude of challenges that must be addressed with precision.
In terms of overcoming these challenges, we have meticulously planned and employed the safest drugs and special apparatus for anesthesia, particularly for reversing the anesthesia state and ensuring the safety of the animal and our team.
Pre Analysis Plan
We plan to provide the mean and range of the veterbral heart scale, cardiothoracic ratio, and NT-proBNP biomarker. Subsequently, we will compare the relationship between VHS, CTR, and NT-proBNP. We will then create age and sex groups to compare the VHS between groups in order to determine if there are any differences. These tests will provide dependable data that can be applied in daily practice.
Protocols
This project has not yet shared any protocols.
