About This Project

Mycoremediation is a form of bioremediation that uses fungi to clean up contaminated sites. Brown rot and white rot are categories of fungi that produce different suites of digestive enzymes that have each shown potential for mycoremediation. We aim to bolster the efficiency of mycoremediation techniques currently in use by combining white rot and brown rot fungi to produce a broader arsenal of digestive enzymes to break down diesel in contaminated soils.

Ask the Scientists

What is the context of this research?

Mycoremediation by wood-decay fungi is often associated with white rot fungi. White rot fungi produce digestive enzymes that preferentially degrade lignin, a component of wood that is broadly similar in molecular structure to petroleum hydrocarbons. In contrast, brown rot fungi degrade the cell wall carbohydrates of wood, and some have been found to use metabolic capabilities not present in white rot fungi to do significant mycoremediation. The ability of white and brown rot fungi to work together to break down complex environmental pollutants has never before been tested.

What is the significance of this project?

Terrestrial oil spills are occurring more frequently in the US. In 2014, crude oil spills by rail increased 18-fold compared to those in 2008. A recent Wisconsin freight train derailment caused a locomotive to leak 1,000 gallons of diesel fuel into the environment. Polycyclic aromatic hydrocarbons (PAHs) found in petroleum products are mutagens and carcinogens of particular concern for both humans and animals. Our goal is to use the findings of our study to improve mycoremediation as a low-cost, effective alternative to traditional and often ecologically disruptive clean-up techniques.

What are the goals of the project?

We aim to determine whether a brown rot and a white rot fungus can persist together when introduced to non-sterile, diesel-contaminated soil; whether degradation of diesel hydrocarbons is greater when both fungi are present compared to either fungus alone; and how the introduction of the fungi to non-sterile contaminated soils affects the composition of the soil microbial community. We will observe pairings of Fibroporia vaillantii (brown rot) and Stropharia rugosoannulata (white rot) using wood block inocula on either ends of trays filled with compressed, contaminated soils. Fungal interaction outcomes will be assessed visually, diesel degradation will be analyzed with GC-MS, and changes to the microbial community will be analyzed with Illumina sequencing.