About This Project

One of the main challenges in applying metagenomics is the capture of cells or residual DNA prior to the extraction process, high-throughput sequencing, bioinformatic and data management. In this study, we aim to evaluate a novel, low-cost, and user-friendly environmental DNA (eDNA) collection method using PVC tubes and plastic bottles filled with positively charged electrostatic nylon (0.45 μm BiodyneTM B, 47 mm), and non-charged cellulose ester (0.45 μm Pall GN-6 MetricelⓇ).

Ask the Scientists

What is the context of this research?

The biodiversity in the ocean, particularly in the West Africa Atlantic Ocean border, is little known. In the scope of the protection of Ocean biodiversity, we would like to apply eDNA approach to study the biodiversity of the Atlantic Ocean. Our project is a contribution to the effort done to explore the biodiversity of the Atlantic Ocean and develop a strategic plan for conservation of biodiversity. For this purpose we are developing a passive DNA collection tool based on PVC tubes connected to a buoy and plastic bottles filled with charged and uncharged nylon filters which can be replaced by polycarbonate or nitrate cellulose filters.

What is the significance of this project?

The project would like to address the concerns related to biodiversity in ocean and particularly the microbiome of ocean. We would like to contribute to global efforts of the protection of biodiversity in Ocean including microorganisms, macroinvertebrates and vertebrates. The application of eDNA approach through metabarcoding and metagenomic offer an important alternative to explore the whole biodiversity in the ocean with limited negative effect on the ecosystems. The development of this passive filtration method can offer the possibility to collect wide range of DNA and then explore the whole biodiversity of the ocean.

What are the goals of the project?

The project aims to develop an affordable and user-friendly filtration method for efficient DNA collection. This method will enable the characterization of biodiversity using metagenomic and metabarcoding approaches. Ultimately, the findings related to metagenomic and metabarcoding will help to propose a strategic conservation plan and biotechnology respectively.