Molecular Work Almost Finished
We have worked through most of our pillbug samples, extracting DNA and doing PCR for CO1 mitochondrial haplotypes and SRDs through 4 primer sets. It has been a lot of work on a school schedule but ...
So, what is this project about...?
First of all, this is a project for high school biotechnology students. In designing the project I wanted to pick an underused biotechnology model in schools (plants), a cool and well known symbios...
PCR Back on Track
After some issues with primers we are back on track. Each pillbug sample will have 5 PCR runs. That's a lot of PCR. From these, three potentially can be sent for sequencing. The COI will be sent fo...
One Hundred Samples Extracted So Far
We have successfully extracted DNA from over 100 pillbugs submitted from Georgia, North Carolina, California and New York. Each sample will have PCR and gene sequence of both the COI mitochondrial ...
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