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Week of 7/25 - 7/31
7/253X Phire Reactions (50ul)Contentsul Phire 25 H2O 23.2 50 uM F Primer 0.5 50 uM R Primer 0.5 95.1ug/ul Construct 0.8 PCR run with 30 cycle...
Week of 7/18 - 7/24
7/18Ran a digest of PEP352GAP vectorContentsExperimental (ul)Control (ul) H2O 19.9 20.9 10x Cutsmart 2.5 2.5 XnoI .5 X PvoII-GF .5 ul X DNA (306.6ug/ul) 1.63 1.63 Mix tubes by flicking...
Week of 7/11 - 7/17
7/11 Ran PCR on the construct (5x) PCR'ed 84.2 ng/ml construct 5 replicates made using Phire Polymerase Used the following program for the thermocycler: Thermocycler ProgramStepTemperature (Degre...
Lab Notes
Hello! Josh here, and I'll be posting data on the experiments we run on notes organized by week. If you have any questions, please let me know!