I have spent several months trying to figure out the best way to recognize and thank the people who had donated to the crowdfunding experiment. I thought of several ideas in the past, such as givin...
We were still unable to detect the expression of recombinant proinsulin in chlamydomonas from our pAsapI construct, despite the fact that the construct seemed to have successfully integrated into t...
We were finally able to detect the algae proinsulin gene. We had re-cultured the emergent transformed microalgal colonies on minimal media to ensure that the colonies contained our pASapI-proinsuli...
It has been an eventful 2016, to say the least. On the technical side, we were able to transform an antibiotic resistance marker successfully into microalgae (pictured below). Unfor...
When we began the IndieBio EU accelerator program, we started experimental work immediately. With the proinsulin and spectinomycin resistance cassettes successfully constructed by Sebastian, and tw...
Last week, we reached another milestone. We were able to successfully confirm that our modular expression system (derived from the pASapI plasmid) is indeed functional, and working well in E. coli....
Thanks to the amazing support and enthusiasm we received from the community through the Experiment.com platform, our team was offered an incredible opportunity to join the IndieBio synthetic biolog...
If you've followed our methods section, you'll be happy to know that we completed some important experiments on our path to making algal insulin a reality. First off, the proinsulin gene was clone...
It has been an incredibly busy few weeks, which is why this overdue update is a bit late. The algal DNA construct is already done but we do not know if it works, so we needed to test in bacteria fi...