Thomas Irvine

Thomas Irvine

Sep 04, 2014

Group 6 Copy 60
1

Still chuggin along

Hello everyone,

          I got some good news today. I have found a CRISPR plasmid that will work in the plants I am using. I'm only waiting for a response from a professor here at UC Davis regarding the tumor causing bacteria. He's probably out of the country for the summer; so I will just have to wait for the beginning of the fall quarter. I will soon have all the pieces I need to make this project successful. 

         Even if I do not reach my funding goal I am still going to go through with this project. This sort of science far is too necessary for me to not to. I am not daunted by the limited, recent interest in my project because I know what I am doing is important. I hope all of you who have backed my project also believe in its importance.

I am going to post an article I wrote on CRISPR in a couple of days, and I hope that it will be very informative to non-experts who are curious about CRISPR. I also hope that it will provide understanding that my project is not a pipe-dream, but in fact the future of how we will treat disease. 

Lastly, I would like to say a BIG thank you to my backers! So THANK YOU!

-Thomas Irvine

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  • Abdullah Farooq
    Abdullah Farooq
    Hey Thomas, I just wanted to say that I truly hope your work succeeds. I had a similar experience with a close family member passing away from cancer while I was in junior high, resulting in me getting into oncological research. In terms of the viral vector, what type of vector system are you looking at? I'd be interested to find out how you are planning on achieving appropriate spread and expression of the system within tumor cells. Also, how are you planning on packaging the system into the vector? I'm struggling with the logistics right now of trying to figure out how to package the Cas9 D10A Nickase and two gRNAs into an oncolytic virus genome - so I'd be interested to know how your tackling the issue. Again, all the best! Abdullah
    Sep 06, 2014
  • Thomas Irvine
    Thomas IrvineResearcher
    Abdullah, At this point, I am not planning on using a paired nickase. I want the payload to be as small as possible to ensure adequate and efficient dispersal of the viral particles. I know I'm not answering your question fully. This is a crowd-sourcing site for experiments that are not fully off the ground, therefore I cannot divulge too much at this point. This is not a grant proposal so I have to be wary about releasing too many details. I have already seen some other experiment pages that have used similar information to what I have posted. Some of my methods must remain proprietary until I publish, and so I have intentionally been scientifically vague or miss leading in certain places. All your answers will be revealed when I publish this open source. Thank you for your interest! If you are really interested, feel free to donate. I will be able to give you the answers you seek sooner! Thomas I.
    Sep 07, 2014

About This Project

Cancer is a disease that affects us all. I believe that genetic editing can be used to halt tumor growth and cease the formation of new tumors in all organisms able to utilize the system, but I need your help. Genetic editing methods, like CRISPR, have been shown to be effective and accurate in plants, mice, zebrafish, and human cells. While this system has been used to edit individual genes, it has not been used in any anti-tumor experiments.

More Lab Notes From This Project

Blast off!

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