Puerto Rico is a U.S. territory with approximately 3.4 million inhabitants that experiences periodic outbreaks of new and emerging mosquito pathogens like dengue, Zika, chickungunya, and West Nile. However, it has been more than 80 years since a complete island-wide survey of mosquitoes has been conducted. Without complete and accurate knowledge of which mosquitoes are present and their distribution, planning effective control efforts and understanding disease dynamics is problematic.