Hastings Reserve is home to three streams that provide critical habitat for sensitive native species. Through the use of eDNA sequencing in stream channels, I hypothesize that I will confirm the presence of two threatened species, the foothill yellow-legged frog (Rana boylii) and Central Coast steelhead (Oncorhynchus mykiss). This will inform conservation planning and habitat restoration, particularly with respect to reducing human impacts on these fragile ecosystems.