Annie Deck

Annie Deck

May 23, 2022

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Research updates

This semester I have completed a variety of DNA extractions using various commercially available kits to test the efficiency and accuracy of each one as it pertains to my samples. After fluorometric methods were used to determine the yields of bacterial DNA extracted, PCR and gel electrophoresis methods began. Once these processes are complete on all of the extractions, they will be sent for 16S rRNA amplicon sequencing funded by the Experiment.com Ocean Solutions grant challenge. By analyzing the sequencing data, which the STAMPS course will be highly informative in, we will determine the optimal DNA extraction protocol for my samples and use that moving forward in the next phases of my research. More updates to come this summer!

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About This Project

Yellow tang are vital to Hawaii’s reef ecosystems, but wild populations have been drastically declining due to the aquarium trade. Aquaculture is a sustainable alternative to removing fish from the wild, but only a fraction of cultured yellow tang survive to adulthood. We suspect investigating the microbiome will provide key insights to this problem. Our goal is to increase the success of the Oceanic Institute's aquaculture program and reduce impact on wild yellow tang populations.

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