Danny Newman

Danny Newman

Mar 24, 2018

Group 6 Copy 348
1

Samples Sent

Ranger Roo with the specimen tubes

At long last, after working out a few final kinks in our specimen database, a box containing 294 tissue samples, suspended in buffer-filled tubes, was sent off for sequencing. All 294 will be sequenced using the ITS1F and LR3 primers, while 71 of those samples will receive additional, multi-locus treatment to look at the RPB2 and β-tubulin gene regions. As stated in the campaign video, we expect a turn-around time of 1-2 months.

A belated but nonetheless special thanks to our lab assistant, Allie Ludden, who not only helped tremendously in the tissue sampling process, but will be staying on with the project to help with specimen repackaging and accessioning for deposit at the Oregon State Univeristy herbarium in Corvallis; our collections' final resting place.

Awesome Allie hard at work in the wet lab

We'll check back in with you all in the next month or two once the results are in. Until then, happy spring and thank you again for all your support!

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  • Nikita Thorat
    Nikita Thorat
    Nice
    Mar 25, 2018

About This Project

Of an estimated 3.2 million species of fungi, only some 120,000 are known to science. Most of the undescribed species reside in the tropics. In 2014, myself and a fellow mycologist, Roo Vandegrift, collected some 350 samples of fungi from Reserva Los Cedros; one of the last unlogged watersheds on the western slope of the Andes. We are now looking to begin the microscopic and molecular analysis portions of our research, with the goal of publishing on our findings.

Blast off!

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