Cultivated seafood research lags behind in large part due to limited data, especially on a single-cell level. Our project aims to fill this gap by generating transcriptomic & epigenomic data using the KFE-5 (killifish) cell line. We'll evaluate variability in long-term proliferation/differentiation potential, and use this data and subsequent bioinformatics analysis to lay the groundwork for optimizing cell line performance, serum free media development, and antibody development.