Rachel Aronoff

Rachel Aronoff

Sep 21, 2023

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More about #mycelia4bees

Not only will we be doing biomaterial workshops (tomorrow and next Thursday) to make a smaller version of the 'fruit bowl' with local school kids, we received our MinION flowcell and the latest reagents for Nanopore sequencing yesterday. This will make a big difference for the metagenomic assessment of biodiversity, in comparison to the 'one by one' sequence results we have to date! One example of this is shown below.

The gel and results for one set of species IDs (genus Cortinarious for B and C, and species crassus for B) from individual samples

Sample E did indeed turn out to be a local 'turkeytail' - Trametes versicolor. Tests of other systems to extract DNA from dirt samples are projected, obviously, given the empty lane D. (Only one dirt sample gave some weak mITS1/4 product, to date...)

The BeeMoS system data communication tests have been promising, as the weather is cooling down (finally). Whether the scale system we are implementing will be robust enough, remains to be seen.

Happily, we are excited to be participating in a special fungal-themed gathering, soon, the BIOFEST Meraki, from 19-22 October in Astros, Greece.. Maybe you can join in? The deadline to propose your contribution(s) is 1 October!

Please check out the pdf linked below.

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  • Rachel Aronoff
    Rachel AronoffResearcher
    The program for the launch of the biofablab in Greece was very ambitious, but we got many good things done! We even left Fomes fomentarius cultures for biomaterials growth, to hopefully be used by the Thyrea beekeepers in participatory research tests. Vive the Thyrea FabLab.
    Nov 01, 2023
  • Rachel Aronoff
    Rachel AronoffResearcher
    It's still very busy times, with not only the latest orders received and metagenomics in November set to happen, but also this project a central focus for both the 'Fête de la science et biodiversité' at Voltaire's old château (14oct23) and the Biofest for the Meraki People Biofablab in Astros, Greece! Here is the link to the presentation planned for that, tomorrow (19oct), a prezi -https://prezi.com/view/vXXahfeh95Tjl2Jy0HYZ/ ! Please let us know if you have any input, comments or still would like to participate. (Really hoping for more mails from beekeepers by the end of October!) Thanks! =)
    Oct 18, 2023
  • Rachel Aronoff
    Rachel AronoffResearcher
    thanks to Yngvar for the spelling fix on that prelim figure! (noted in the legend)
    Sep 26, 2023

About This Project

Of the millions of fungal species, less than 150,000 are known. At our community lab, Hackuarium, we have already used DNA sequencing analyses in several projects (eg. BeerDeCoded, OpenFoodRepo DNA) and have begun to apply these methods to investigate fungal biodiversity. Now, with our mushroom expert, Yngvar, we propose further metagenomic analyses, and to also explore mycelial feeding to honey bees in the context of another lab project, BeeMoS, to possibly protect them from pathogens.

Blast off!

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