Dylan Paoletti
101 N Haven St Baltimore, MD 21224
Baltimore Under-Ground Science Space
Principal Investigator
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UPDATE
Big thanks to all those who have pledged donations for this project!Unfortunately we didn't reach our goal. However, we plan to continue the research in different ways and with more affordable mode...
PROJECT UPDATE
Me (Dylan Paoletti) and my Mentor (Dr. Peter Evans) are pursuing expansion of the project by founding a Start-Up Company (Plasmid Therapeutics). We hope to get investments from accelerator and incu...
Steps
The phase 1 project can be broken down into these steps: Order DNA from IDT (approx 10.6kb) Phosphorylate and ligate blunt ends (use NEB SDM kit) Transformation DH5 alpha cells, plate on LB + 100 u...