Yuriy Fazylov

"CRISPR experiment involving gene editing to determine the maternal to zygotic transition in rice" details? were you going to simply cut out the genes or repress them somehow?

This is just an assumption. With all the medical application of either technology your aspirations seem to be therapeutic rather than plant based. I don’t understand why not just explain that you can’t work with animal cells at current time and that plants are the next best thing. Why draw parall...more

Excuse my curiosity. I didn't realize I hit intellectual property questions so soon.

No problem. I was interested in using CRISPR-Cas9 on plants myself, mostly as recombinant Cas9. What kind of guide RNA will you have? sgRNA or crRNA + tracrRNA? Have you tried looking into; if the Ti vector already has a spacer associated with it in agro or other bacteria that speedily does aw...more

what agro and Ti vectors will you be using? wt or lab cultured? how will you know if there is a diversity in Ti vectors? what is your gRNA target? T-DNA itself?

holy cow. you just gave me an idea.

Thank you. Your will be appreciated.

I don't like not knowing. http://cosmiclog.nbcnews.com/_news/2007/01/05/4350893-lost-cities-seen-from-space http://www.pbs.org/wgbh/nova/ancient/maya.html CaCO3 seems to be associated with the answer to your question. Look for a plant that would allow for high amount of Calcium to build...more

>A crazy idea. But why not use Cyan Fluorescent Protein instead of melanin? Yes crazy idea, mainly because of limitations in stokes shift activity of CFP. It gets excited not even at long, UV-A, but violet 435 nm - and emits back at blue 485 nm. Those are its peak activities. It doesn’t do a v...more