In two isolated "sky islands" on the island of Trinidad live an IUCN critically-endangered species, the golden tree frog. We know little about the golden tree frog, so it is very difficult to help protect this rare species. It is nationally and internationally recognized as threatened and important for conservation and cultural reasons. To understand this endangered species we need to develop two new non-invasive methods to study them: environmental DNA (eDNA) sampling and vocalization surveys.