Exome-capture: why and how?

Why:

In some cases, sequencing the entire genome of an individual is the best way to collect DNA sequence for a study.  By now, most of us of heard about the virtues and medical promise of sequencing human genomes.  In many cases, however, full genome sequencing may not be the best choice.  Full genome sequencing can be problematic because...

1. It's expensive

2. It requires previous genomic resources

3. The content of the genome may be repetitive and problematic to study.

All three of these issues make it more efficient and more sensible (for now) to seek alternatives to genome sequencing for studies of Aedes albopictus (the Asian Tiger mosquito). Instead, we're going to use an innovative genomic approach to sequence only the parts of the genome that make proteins - the protein coding genes, or the exome.  

How:

To sequence the exome, we're designing a custom set of short DNA molecules (probes) that are carefully chosen to hybridize or stick to the protein coding genes in the (target) genome.  The figure below gives a pictorial description of the protocol for the NimbleGen technology that we'll use.   

 

We're very excited to use this technology in a system where we know very little about the genetics of the organism.  Sequencing just the protein coding genes in the genome is very economical, efficient, and informative.  In one experiment, we will get a genomic view of how the genetics of organisms has changed and adapted since they moved out of South East Asia.  

Join us in moving this system into the Genomic Era and help us find new ways to control a remarkably successful invasive, disease-transmitting mosquito!!

Visit my experiment webpage!!