The midwife toad (Alytes obstetricans) is an alien species, first introduced to the UK over 100 years ago. It has long been assumed that all of the known populations have a French origin, being sourced from the original founding population in Bedford. Whilst carrying out studies on the Cambridge population, it was determined that the toads there originated from Spain. Now we aim to sequence DNA from up to 100 toads, ≥5 toads from 20 known populations to build a haplotype map