Regions of Bali Strait, Indonesia, have often experienced a dominant catch of Sardinella lemuru in the last decades [1,2,3], which benefits the economy & ecology aspects. However, where it went during the rising La Nina intensity in the climate change era [4] with morphological sample scarcity, this study proposes new sampling to increase eDNA recovery of S.lemuru by increasing the previous standard filtered water [11,12,18] to 3-10 L triplicate in 3 & 15 m depth without adding high field cost.