Mycoplasma bovis (M. bovis) is an economically important bacterial pathogen of cattle. We recently documented seasonal occurence of M. bovis in pronghorn antelope in northeast Wyoming in 2019 and 2020, resulting in over 500 mortalities. Through targeted surveillance, we aim to determine if exposed pronghorn serve as carriers of M. bovis that pose a transmission risk to naive wildlife and livestock. We expect to find reservoir pronghorn in the previously exposed population.