10/13 Au-NP conjugates DTNB
Au-NP : 10 nm in 0.1mMPBS. (5X 10^12 Particles/ml)
DTNB solution:10mM in EtOH
Procedure: 1. 1ml Au-NP +10ul DTNB solution.
2. Rotation overnight in RT.
3. Centrifuge 13000rpm, 1hour at 4℃.
4. Wash 2 time with water.
5. Suspension with 1ml water.
6. Measured absorbance value.
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