Jiunyi Shaw

Jiunyi Shaw

Oct 13, 2017

Group 6 Copy 68
0

10/13 Au-NP conjugates DTNB

Au-NP : 10 nm in 0.1mMPBS. (5X 10^12 Particles/ml)

DTNB solution:10mM in EtOH

Procedure: 1. 1ml Au-NP +10ul DTNB solution.

2. Rotation overnight in RT.

3. Centrifuge 13000rpm, 1hour at 4℃.

4. Wash 2 time with water.

5. Suspension with 1ml water.

6. Measured absorbance value.

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About This Project

M. tuberculosis is slow growing pathogen, TB in early infection is difficult to diagnose correctly, so culture is the WHO-recommended gold standard for TB diagnosis, but it may take more than 1 to 3 months. The patient may inadvertently become a spreader while waiting for the results. We propose a solution using Raman spectroscopy that can detect pathogen more accurate than the current test. If our results are positive, we should be able to early detect and treat TB to prevent spread of disease.

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