I will study the effects of three types of sustainable carbon-based media on the biobutanol yield of two types of bacteria in a small bioreactor. Butanol has much potential, as it can be used in combustion engines designed for gasoline and has beneficial fuel properties compared to ethanol. The Clostridium genus has two species (C. acetobutylicum and C. beijerinckii) that can ferment butanol. Glucose is easily digested in respiration, so I predict it will be the most efficient in my reactor.