Mfritts

Mfritts

Feb 17, 2025

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Construct Information

Here is the breakdown for each Construct and what each part does

This igfbp1a knockout construct introduces indels into the first exon of igfbp1a, effectively disrupting gene function while also expressing green fluorescence for visualization.

https://en.vectorbuilder.com/pdf/vector/VB250217-1538vda.pdf



This RHEB overexpression construct stimulates mTOR activation to promote hypertrophy, enhance muscle growth, and make the fish fluoresce red due to mCherry, a red fluorophore.

https://en.vectorbuilder.com/pdf/vector/VB250217-1533huv.pdf


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About This Project

What if we built a better fish instead of depleting wild fisheries? Natural stocks can’t keep up with demand, making aquaculture essential. Yet, farmed fish struggle to compete with the profitability of exploitative fishing. By restricting growth inhibitors and stimulating key developmental pathways in zebrafish, I aim to develop a methodology for producing faster-growing, more efficient fish creating a foundation for genetically optimizing aquaculture species.

More Lab Notes From This Project

Blast off!

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