End of the Field Season!

 

Genspace Microbial Reef Project Members with Billion Oyster Project at Bush Terminal Piers Park

Hello there! We've made great progress over the past five months since we have received the grant in July.

From July to September, we have sampled water from Bush Terminal Piers Park, and extracted the DNA from the filtered samples using the Qiagen DNEasy PowerWater kit. In October we sent our DNA samples for sequencing using the NovaSeq XP with paired end sequencing. And now, WE HAVE DATA!

 

Tanasia from Billion Oyster Project explaining the history of the reef to Genspace members

 

Water samples from Bush Terminal Piers Park

 

Genspace members extracting DNA using the Qiagen DNeasy PowerWater Kit

 

Quantifying the DNA concentration of our extracted DNA for sequencing.

 

Table of DNA samples we sent for sequencing to Novogene for sequencing

 

Screenshot of terminal after downloading our data from 15 samples and a keepsake 3D printed bookcase holding the memory card with our dataset

Just this week we got access to Ookami, an HPC environment based at Stony Brook University https://www.stonybrook.edu/ookami/ . We're very excited to analyze our dataset to gain a better understanding of the microbiome of our local oyster reef!