The Florida Keys are essential nursery habitat for commercial and recreational fish species. Despite this vital ecologic and economic position, studies reveal significant plastic contamination, highlighting a critical knowledge gap in ultra-small (<0.3mm) concentrations, which pose significant risk to ecosystem health[1]. We will fill this gap with first-of-a-kind collection of 0.3-0.05mm plastic using our unique trawl system, initiating long-term mitigation and monitoring projects.