Recent trials in the Galapagos demonstrate that environmental DNA (eDNA) is a powerful biodiversity monitoring tool. This project compares PUF filters (emerging technology) and cellulose membranes (industry standard) using diver-towed devices at four sites in Monterey Bay National Marine Sanctuary. One filter will emerge from the trial as having outperformed the other in capturing biodiversity, enabling the development of standardized marine eDNA monitoring protocols.