Cancer spread begins when invasive cells cross the basement membrane, but current models fail to capture this step. To overcome this, we will engineer a basement membrane using Human Umbilical Cord-derived Mesenchymal Stem Cells (UC-MSCs) over a collagen matrix and quantify invasion. We hypothesize that Myh9, a protein that generates cellular mechanical forces, promotes cancer cell invasion across the basement membrane. This model can also be used to study other regulators of invasion.