The usual practice on all commercial farms in the egg-laying industry is to cull male chicks shortly after hatching since they cannot lay eggs. Worldwide, an estimated 7 billion day-old male chicks are culled annually. Engineering an all-female breed of egg-laying chickens could put a stop to this unethical practice. This project aims to identify testes-specific enhancers for DMRT1 and HEMGN – an essential first step in creating an all-female breed of chickens.