Behind the Lab Bench: How we go from organoids to imaging (cont.)

The final and the most exciting part of the process..... staining and imaging. After we got super thing sections of the tissues, we have to process them to get rid of the wax so they are ready for staining. Here we used hematoxylin and eosin staing (aka H&E staining). Hematoxylin stains the nucleus blue while eosin gives a pink hue to the space inside the cells and between cells.

Once the tissues are all nice and colorful, we examine them under a microscope to study their structure and morphology. Pathologists use this very same procedure to diagnose different diseases in clinics as well.

This is one of many stainings we can do to study the tissues and organoids. We can even do fluorescent staining to locate different proteins or genetic materials inside the cells. Those are always pretty. -Tee