Additional contents of the project goal

These are the key goals for this project:

Aim 1: Select agricultural waste to extract peptides and the methods.

Approach 1: Research papers on agricultural waste containing peptides and growth factors similar to the cultivated meat cell culture media were investigated and experimental groups were selected.

Approach 2: Disscus how to extract peptides from agricultural waste (with sustainable and green methods) using water based methods, acids, enzymes and related approaches.

Expected outcome: When selecting experimental groups from agricultural waste, they are appropriately distributed in cerals, grains, fruits, and marine life.

Aim 2: Peptides from the agricultural waste are utilized in cell culture to monitor and evaluate the extent to which they improve cell replication, and then cell differentiation. 

Approach 1: Peptide sequences will be mapped from currently used growth factors; including identified bioactive peptides in previous research.

Approach 2: Matching peptides (sequences) for known growth factors of scientific interest for cell growth and differentiation (accessible database comparing the sequences). 

Approach 3: The peptides will generated will be characterized to build the database

Expected outcomes : growing muscle cells in culture with cell ag-derived  peptides to see how they improve (or not) cell replication and cell differentiation.

This research presents a unique window of opportunity for us to explore a way forward to produce growth factors necessary for sustainably grown cutivated meat that do not harm the environment and are available from low cost agricultural waste materials. At a global scale, this project is a win-win scenario as, while directly contributing to SDG #2, it can help reduce the associated costs involved in cultivated meat production.