Aug 01, 2017
E. coli integrated various regions of D. scabra genome and DNA fragments into their plasmids. I amplified that DNA, and ran the product on an electrophoresis gel. The ingrated DNA can be seen as different sized bands on the gels! Hopefully we will see some more microsatellites when we send these clones out for sequencing!
Tina, ReneeMBaudoin, and 1 other like this.
Dubautia scabra is a native plant on the Hawaiian islands, and is the first flowering plant on new lava flows. I am investigating genetic and ecological patterns of colonization and succession of D. scabra on lava flows to understand mechanisms of plant colonization. I use leaf tissue as the DNA source to analyze evolutionary histories. This data helps us understand how plants colonize new habitats with direct implications for understanding responses to climate change.
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